Journal: AMB Express

Article Title: Glycerol conversion to 1, 3-Propanediol is enhanced by the expression of a heterologous alcohol dehydrogenase gene in Lactobacillus reuteri

doi: 10.1186/2191-0855-1-37

Figure Lengend Snippet: Primers and peptide sequences used in this work

Article Snippet: Primers were procured from VBC-Biotech (Austria) and the inducer sakacin P induction peptide (SppIP) was synthesized from GenScript (USA).

Techniques: Sequencing

Journal: AMB Express

Article Title: Glycerol conversion to 1, 3-Propanediol is enhanced by the expression of a heterologous alcohol dehydrogenase gene in Lactobacillus reuteri

doi: 10.1186/2191-0855-1-37

Figure Lengend Snippet: SDS-PAGE analysis of L. reuteri whole cell lysates for yqhD expression . Lane 2 untransformed L. reuteri , lane 3 uninduced recombinant L. reuteri HR2, lane 4 recombinant 5 h after induction with SppIP, lanes 1 & 5, protein molecular weight marker.

Article Snippet: Primers were procured from VBC-Biotech (Austria) and the inducer sakacin P induction peptide (SppIP) was synthesized from GenScript (USA).

Techniques: SDS Page, Expressing, Recombinant, Molecular Weight, Marker

Journal: Bioactive Materials

Article Title: A ROS-responsive hydrogel incorporated with dental follicle stem cell-derived small extracellular vesicles promotes dental pulp repair by ameliorating oxidative stress

doi: 10.1016/j.bioactmat.2024.06.036

Figure Lengend Snippet: DFSC-sEVs enhanced the survival, proliferation and osteogenesis/odontogenesis of H 2 O 2 -damaged DPSCs. (A) DPSCs apoptosis was examined by an Annexin V-FITC/7AAD assay. (B) Apoptosis rates of DPSCs, including early apoptosis (Annexin-V + 7AAD − ) and late apoptosis (Annexin-V + 7AAD + ). (C, D) Representative images of BrdU staining and quantification of DPSCs. (E) Representative images of alizarin red staining after osteogenic induction for 21 d. (F) qRT‒PCR analysis of the mRNA expression of the odontogenesis-related factors Alpl , Ibsp and Dcn . (G) Representative images of Oil Red O staining after adipogenic induction for 21 d. (H) qRT‒PCR analysis of the mRNA expression of the adipogenic-related factors Cfd , Cebpa and Cebpb . *** P < 0.001, ** P < 0.01, * P < 0.05.

Article Snippet: The osteogenic differentiation of DFSCs and DPSCs was induced with osteogenic induction media for 21 days according to the manufacturer's instructions (Cyagen Biosciences, China), after which alizarin red staining was performed to visualize calcium deposits.

Techniques: BrdU Staining, Staining, Expressing

Journal: Bioactive Materials

Article Title: A ROS-responsive hydrogel incorporated with dental follicle stem cell-derived small extracellular vesicles promotes dental pulp repair by ameliorating oxidative stress

doi: 10.1016/j.bioactmat.2024.06.036

Figure Lengend Snippet: DFSC-sEVs exerted cytoprotective effects on H 2 O 2- induced damage by maintaining mitochondrial homeostasis. (A) DPSCs apoptosis was examined using an Annexin V-FITC/7AAD assay. (B) Apoptosis rates of DPSCs, including early apoptosis (Annexin-V + 7AAD − ) and late apoptosis (Annexin-V + 7AAD + ). (C, D) Representative images of BrdU staining and quantification of DPSCs. (E) Representative images of alizarin red staining after osteogenic induction for 21 d. (F) qRT‒PCR analysis of the mRNA expression of the odontogenesis-related factors Alpl , Ibsp and Dcn . (G) Representative images of Oil Red O staining after adipogenic induction for 21 d. (H) qRT‒PCR analysis of the mRNA expression of the adipogenic-related factors Cfd , Cebpa and Cebpb . *** P < 0.001, ** P < 0.01, * P < 0.05.

Article Snippet: The osteogenic differentiation of DFSCs and DPSCs was induced with osteogenic induction media for 21 days according to the manufacturer's instructions (Cyagen Biosciences, China), after which alizarin red staining was performed to visualize calcium deposits.

Techniques: BrdU Staining, Staining, Expressing

Journal: Journal of Orthopaedic Translation

Article Title: Sustained notch signaling inhibition with a gamma-secretase inhibitor prevents traumatic heterotopic ossification

doi: 10.1016/j.jot.2023.06.004

Figure Lengend Snippet: TMPCs expressed stromal cell markers and exhibited the chondrogenic and osteogenic differentiation potentia l (A) The morphology of TMPCs (B) The specific surface markers of TMPCs analyzed by flow cytometry (n ​= ​3) (C) Safranin-O staining of TMPCs pellets under a chondrogenic induction medium over 21 days (n ​= ​3) (D) Alizarin red staining of TMPCs under an osteogenic induction medium over 21 days (n ​= ​3). Scale bar ​= ​100 ​μm. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: The chondrogenesis and osteogenesis were induced by chondrogenic or osteogenic induction medium (Cyagen) for mouse BMSC.

Techniques: Flow Cytometry, Staining

Journal: Journal of Orthopaedic Translation

Article Title: Sustained notch signaling inhibition with a gamma-secretase inhibitor prevents traumatic heterotopic ossification

doi: 10.1016/j.jot.2023.06.004

Figure Lengend Snippet: Sustained administration of DAPT represses TMPCs osteogenic differentiation in vitro (A) Alizarin red staining of TMPCs after osteogenic induction in control, 5-day and 14-day DAPT groups (B) Quantification of Alizarin Red S positive areas in different groups (C) Western blot analyses of the expressions of osteogenic-specific markers during TMPCs osteogenic differentiation (D–F) Quantification of the expressions of osteogenic-specific markers assessed by western blot (G) Western blot analyses of the expression of osteogenic-specific marker and Notch target genes during TMPCs osteogenic differentiation (H, I) Quantification of the expressions of Notch target genes assessed by western blot. ∗ P ​< ​0.05, ∗∗ P ​< ​0.01, ∗∗∗ P ​< ​0.001. Scale bar: 100 ​μm. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: The chondrogenesis and osteogenesis were induced by chondrogenic or osteogenic induction medium (Cyagen) for mouse BMSC.

Techniques: In Vitro, Staining, Control, Western Blot, Expressing, Marker